Group 2

Group 2

Experiment 5 : Content of Ibuprofen (assay)

Objective

To identify the content of ibuprofen whether it tally to the value stated

Apparatus 

i)                    Beaker  50 ml and 100 ml
ii)                  Burette
iii)                Conical flask
iv)                Glass rod
v)                  Hair dryer
vi)                Measuring cyclinder 100 ml and 10 ml
vii)              Electronic balance
viii)            Weighing boat
ix)                Filter funnel

Materials

i)                    Ibuprofen tablets
ii)                  Chloroform
iii)                Ethanol(96%)
iv)                0.1 M sodium hydroxide
v)                  Phenolphthalein

Experimental Procedure

1.20 Ibuprofen tablets  are previously selected at random and weighed and are crushed into powder.

2. A quantity of powder containing 0.5g ibuprofen is extracted and mixed with 20ml
chloroform for 15 minutes.Then, it is filtered through a sintered glass crucible (BS Porosity No.1).

3. The residue is washed with 3 x 10ml chloroform and the combined filtrate is gently evaporated just to dryness in a current of air. The residue is dissolved in 100ml with ethanol (96%) that is previously neutralized to phenolphthalein solution.

4. The solution is then titrated with 0.1M sodium hydroxide to end point with phenolphthalein solution as the indicator. The content of ibuprofen is calculated if each ml of 0.1M sodium hydroxide is equivalent to 0.02063g of  C13H18O2.

Results And Calculations

i)                     1 tablet of Ibuprofen     = 200mg
            20 tablets of Ibuprofen   = 20 x 200mg
                                                    = 4000mg(4g)
          Mass of ibuprofen powder = 7.84 g
         with diluents(20 tablets)
                                          
  
ii)                  To obtain 0.5 g ibuprofen active ingredient,the equation of total mass needed:
                            
                       X= 7.84 x 0.5/4.0
                          = 0.98g

iii)                Mass of ibuprofen powder weighed            = 0.9803 g
Volume of  NaOH used to reach end point = 21.1 ml
Number of mole of NaOH used,n                = 2.11 x 10-3 mole
Molar mass of ibuprofen                              = 206.3 gmol-1

iv)                 

NaOH

C13H18O2
Mole
1x10-4

1x 10-4
Mole Ratio
1

1


v)                            Given n                          = 2.11 x 10-3
          Mass of ibuprofen react = 0.435 g
     
          Standard deviation         = 0.435/0.5  X 100
                                                 = 87 %

Discussion

           
            Based on the result, the standard deviation of the experiment  is calculated by using the formula below:

       Standard deviation = (Experimental value )/(Theoretical value)  ×100%
                                      = 0.435/0.5 × 100%
                                      = 87 %

            From the calculation, standard deviation obtained is 87%. The standard deviation is within 85% to 115% which is based on the range of the British Pharmacopoeia. Thus, the experimental value is considered comply the test. The resulted deviation may be due to several errors which occurred when the experiment was carried out. These errors affect the accuracy of the experiment data.
Some of the errors are the ibuprofen that used for the experiment may have expired which causing the loss of active ingredients in the tablets as the active ingredients might have decomposed. In order to increase the accuracy of the experiment,  ibuprofen tablets that are not expired should be used.
Besides, there also may be  loss of the active ingredients through the crushing process. When  the tablets was crushing into the powder form using mortar and pestle, some of them would drop out of the pestle, causing the loss of ingredients. Thus, to prevent this to happen, the tablet should be crush gently so that the loss of the active ingredient can be reduced.In addition, over-titration would have occurred when carrying out titration with the sodium hydroxide. This is due to the titration is too fast that we missed the volume of sodium hydroxide that caused the first change of colour in the solution. Thus, it would better that if the titration carried out slowly drop by drop which will prevent  from missing the first end point of the titration.


Conclusion

            The ibuprofen content obtained from the experiment is 0.435g, which contributes to the standard deviation of the experiment is 87%, complying the test of uniformity of content which is within 85% to 115%.

References





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